The hosts dominated early as Jorge Recalde scored either side of a Carlos Sanchez penalty at the Manuel Ferreira stadium in Paraguayan capital Asuncion.
Messi was in the headlines on Wednesday after an interview in which he apologized to Barca fans for requesting to leave the club over the summer saying he "accepted" his "error" in an attempt to bring some further calm after a difficult few months at the club.
Also on Tuesday, men's seventh seed Matteo Berrettini of Italy cruised to a 6-3, 6-1, 6-3 victory over Canadian Vasek Pospisi.
In Saturday's other fixtures, Atalanta dominated Torino 4-2, while newly-promoted Benevento stunned Sampdoria 3-2. Enditem
The Viola fought back to restore the lead as Gaetano Castrovilli finished with a low-strike in the 57th minute, six minutes before Federico Chiesa finished from Franck Ribery's assist.
Monday sees Fulham at home while Liverpool entertain Arsenal in arguably the most interesting match of the round. Enditem
Persepolis will meet Pakhtakor in the quarter-finals at the Jassim Bin Hamad Stadium on Wednesday.
The Portuguese club have confirmed the move, which will see Nicolas Otamendi move in the opposite direction.
However, that goal only served to pique Liverpool and Sadio Mane drew them level with a simple volleyed finish after Leno did well to parry a shot from Mohamed Salah on the break.
GWM earlier told the Thai media that it aims to make Thailand its ASEAN (the Association of Southeast Asian Nations) production base for domestic sales and export of new energy vehicles and internal-combustion engine models.
Luo, 20, defeated Billy Castle 3-1 and swept Jamie Wilson 3-0 before locking horn with world number five Mark Allen in his last group match. A draw was enough for the Chinese youngster to book a place in the second stage as the group winner.